Clone

Target
IDP98885  
Clone Suffix
102
Sequence Comments
IDP98885.3-157.P(165).M1_G2del,G158_S165delInsAYTRYVDNNF.pMCSG53  
Experiment Start Date
Nov 22, 2021  
Experiment End Date
Nov 22, 2021  
Person
Michael Endres  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
failed  
Vector
pMCSG53  
Sequence Start
3  
Sequence End
157  
Reference Sequence Used
WDLTVKMLAG NEFQVSLSSS MSVSELKAQI TQKIGVHAFQ QRLAVHPSGV ALQDRVPLAS QGLGPGSTVL LVVDKCDEPL SILVRNNKGR SSTYEVRLTQ TVAHLKQQVS GLEGVQDDLF WLTFEGKPLE DQLPLGEYGL KPLSTVFMNL RLRGG  
PCR Forward Primer
TACTTCCAATCCAATGCCGGTTGGGATTTAACTGTGAAGATGCTTG  
PCR Backward Primer
TTATCCACTTCCAATGTTAGAAGTTGTTATCGACATAGCGAGTGTAT  
Shipped To Lab
ANL  
Shipped Date
Dec 09, 2021  
Inserted
Nov 29, 2021  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2021-09-01 2021-09-03 Christine Tesar Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 0.5 mM IPTG production
2021-12-09 2021-12-09 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB 1 mM IPTG assay