Clone

Target
IDP02136  
Clone Suffix
1
Experiment Start Date
Apr 20, 2009  
Experiment End Date
Apr 20, 2009  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
105  
Reference Sequence Used
MAEWNGEYVS PYAEHGKKSK QVKKITVSIP LKVLKILTDE RTRRQVNNLR HATNSELLCE AFLHAFTGQP LPNDEDLRKE RSDEIPEAAK ILMRELGVDP DTWEY  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MAEWNGEYVS PYAEHGKKSK QVKKITVSIP LKVLKILTDE RTRRQVNNLR HATNSELLCE AFLHAFTGQP LPNDEDLRKE RSDEIPEAAK ILMRELGVDP DTWEY  
PCR Forward Primer
TACTTCCAATCCAATGCGATGGCTGAGTGGAACGGCG  
PCR Backward Primer
TTATCCACTTCCAATGTCAATATTCCCAGGTGTCAGG  
Shipped To Lab
UT  
Shipped Date
Jul 20, 2010  
Inserted
Apr 21, 2009  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2009-04-29 2009-04-30 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay
2011-08-23 Maite Garcia University of Calgary Expression_protocol_Toronto success E. coli 15N 2X M9 none production
2010-05-24 2010-05-25 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay