Clone

Target
IDP99031  
Clone Suffix
102
Sequence Comments
IDP99031.1-186.F..pMCSG53  
Experiment Start Date
Nov 22, 2021  
Experiment End Date
Nov 22, 2021  
Person
Michael Endres  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
failed  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
186  
Reference Sequence Used
MTDALHVKLR PLEREDLRFV HQLDNNASVM RYWFEEPYEA FVELSDLYDK HIHDQSERRF VIECNGDKAG LVELVEINHV HRRAEFQIII SPDFQGKGLA TRAARLAMDY GFTVLNLYKL YLIVDKENEK AIHIYRKLGF MVEGELIHEF FINGEYRNTI RMCLFQHQYL AEHKTPGPSL LKPTAQ  
PCR Forward Primer
TACTTCCAATCCAATGCCATGACCGACGCGCTACACGTC  
PCR Backward Primer
TTATCCACTTCCAATGTTACTGAGCCGTCGGCTTCAGCA  
Shipped To Lab
ANL  
Shipped Date
Dec 09, 2021  
Inserted
Apr 11, 2022  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2022-01-26 2022-01-28 Natalia Maltseva Argonne National Laboratory standard expression_ANL failed E. coli LB 0.5 mM IPTG production
2021-12-09 2021-12-09 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB 1 mM IPTG assay