Clone

Target

IDP99014  

Clone Suffix

105

Sequence Comments

IDP99014.1-184.F..pMCSG53  

Experiment Start Date

Aug 15, 2022  

Experiment End Date

Aug 15, 2022  

Person

Michael Endres  

Lab

Argonne National Laboratory  

Protocol ID

standard cloning_ANL  

Status

failed  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

184  

Reference Sequence Used

MIKNLLKGSN VFLTSFKEEH LNYLEKWYND ILFLRNFDVT AAFPKSLEEL KSIIKNSKES SDKFIFAVNS IRQNKIIGIT GFENILWNNG TATIYIGIGD DDFRGHGIGQ ESLYLTMEFG FQELNFHRIQ LTVLSYNTPA IKLYEKLGFK KEGTYREFIH RDGIRYDMYL YGILRSEWKL NTKY  

Reference Sequence Variance

 

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCCTTGATTAAAAATTTGCTGAAAGGTTCTAATGTATTTCTTA  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTATTTTGTATTAAGTTTCCATTCTGATCTTAGTATG  

Shipped To Lab

ANL  

Shipped Date

Aug 15, 2022  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 29, 2022