Clone

Target
IDP99014  
Clone Suffix
107
Sequence Comments
IDP99014.24-181.P(184)..pMCSG53  
Experiment Start Date
Aug 15, 2022  
Experiment End Date
Aug 15, 2022  
Person
Michael Endres  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
failed  
Vector
pMCSG53  
Sequence Start
24  
Sequence End
181  
Reference Sequence Used
LEKWYNDILF LRNFDVTAAF PKSLEELKSI IKNSKESSDK FIFAVNSIRQ NKIIGITGFE NILWNNGTAT IYIGIGDDDF RGHGIGQESL YLTMEFGFQE LNFHRIQLTV LSYNTPAIKL YEKLGFKKEG TYREFIHRDG IRYDMYLYGI LRSEWKLN  
PCR Forward Primer
TACTTCCAATCCAATGCCCTGGAAAAATGGTATAATGACATATTATTTCTAAGAAACT  
PCR Backward Primer
TTATCCACTTCCAATGTTAATTAAGTTTCCATTCTGATCTTAGTATGCCATAAA  
Shipped To Lab
ANL  
Shipped Date
Aug 15, 2022  
Inserted
Aug 29, 2022  

Related Expressions

 
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2022-08-15 2022-08-15 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB 1 mM IPTG assay