Clone

Target
IDP99017  
Clone Suffix
110
Sequence Comments
IDP99017.1-154.F..pMCSG53  
Experiment Start Date
Aug 15, 2022  
Experiment End Date
Aug 15, 2022  
Person
Michael Endres  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
failed  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
154  
Reference Sequence Used
MSDDDVDNVI EVARDSWKWT YKDIYSDEFI ENWISEKYSK EKLLNEITKS QLNLDLVFLG SFHESKLIGF IELKISSSKA ELLRLYLRPE YTHRGVGRIL LSEAEELVKK RDVEECILYV HKKNYIATSF YKKNGFQVKD IYGDDLIMIK RYKV  
PCR Forward Primer
TACTTCCAATCCAATGCCTTGAGTGATGACGATGTTGATAACGTGATT  
PCR Backward Primer
TTATCCACTTCCAATGTTAAACTTTGTATCTCTTTATCATGATAAGATCGTCG  
Shipped To Lab
ANL  
Shipped Date
Aug 15, 2022  
Inserted
Aug 29, 2022  

Related Expressions

 
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2022-08-15 2022-08-15 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB 1 mM IPTG assay