Clone

Target

IDP02500  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

43: F -> S  

Experiment Start Date

Apr 20, 2009  

Experiment End Date

Apr 20, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

192  

Reference Sequence Used

MTQEIEIEFK NIVTEEEFHA LCKSFSIEVF TKQVNHYFET PNFSLKEAGS ALRIRHKGET YTLTLKQPAE VGLLETHQVV TENEAKMMME TNVIISGAVM NQLCKLQIPV SALTYMGSLT TERAETLFEG GTLVFDHSFY YNHDDYEIEF EVQDEETGKA AFIHLLKQHN IPIRHTNNKV KRFFLAKQNK AR  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MTQEIEIEFK NIVTEEEFHA LCKSFSIEVF TKQVNHYFET PNSSLKEAGS ALRIRHKGET YTLTLKQPAE VGLLETHQVV TENEAKMMME TNVIISGAVM NQLCKLQIPV SALTYMGSLT TERAETLFEG GTLVFDHSFY YNHDDYEIEF EVQDEETGKA AFIHLLKQHN IPIRHTNNKV KRFFLAKQNK AR  

PCR Forward Primer

TACTTCCAATCCAATGCGATGACACAAGAAATTGAAATTGAA  

PCR Backward Primer

TTATCCACTTCCAATGTTAGCGTGCTTTGTTTTGTTTG  

Shipped To Lab

ANL  

Shipped Date

May 04, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Apr 21, 2009