Clone

Target
IDP00260  
Clone Suffix
1
Experiment Start Date
Apr 20, 2009  
Experiment End Date
Apr 20, 2009  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
176  
Reference Sequence Used
MDSRIAIYVL VSASLLYLVN CHKLVHYFNL KINGSDITNT ADILLDNYPI MTFDGKDIYP SIAFMVGNKL FLDLYKNIFV EFFRLFRVSV SSQYEELEYY YSCDYTNNRP TIKQHYFYNG EEYTEIDRSK KATNKNSWLI TSGFRLQKWF DSEDCIIYLR SLVRRMEDSN KNSKKT  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGGATTCGCGTATAGCTATTT  
PCR Backward Primer
TTATCCACTTCCAATGCTAAGTTTTTTTACTGTTTTTGTTA  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2008-08-25 2008-08-26 Chris Nelson Washington University WU_native_soluble_expression_1 success E. coli Athena_SeMET IPTG production
2009-04-29 2009-04-30 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2010-05-24 2010-05-25 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay