Clone

Target

IDP01700  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Apr 27, 2009  

Experiment End Date

Apr 29, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

193  

Reference Sequence Used

MGGKLRIIGG KWRSRKIDFP DLPDLRPTTD RIRETLFNWL APYIVDANCL DLFAGSGALG FEALSRGAAQ VTFVDHSRYV VDKLRKNVEI LQATNAQVLC GDFTSVIPFL TTTSYNIVFL DPPFYKNLVE RAALHLEESK LLAPNAFIYV ETEKNLKPLP LPKTWKIYRE KATSSLAYYL FQHLPQKKGV EKG  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGGCGGTAAACTAAGAATC  

PCR Backward Primer

TTATCCACTTCCAATGTCACCCTTTTTCAACACCTTTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 05, 2009