Clone

Target
IDP01842  
Clone Suffix
1
Experiment Start Date
Apr 29, 2009  
Experiment End Date
Apr 30, 2009  
Person
Sam Light  
Lab
Northwestern University  
Protocol ID
NU_native_expression  
Status
success  
Vector
MCSG7  
Sequence Start
1  
Sequence End
438  
Reference Sequence Used
 
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MDYQTIPSQG LSGEICVPGD KSISHRAVLL AAIAEGQTQV DGFLMGADNL AMVSALQQMG ASIQVIEDEN ILVVEGVGMT GLQAPPEALD CGNSGTAIRL LSGLLAGQPF NTVLTGDSSL QRRPMKRIID PLTLMGAKID STGNVPPLKI YGNPRLTGIH YQLPMASAQV KSCLLLAGLY ARGKTCITEP APSRDHTERL LKHFHYTLQK DKQSICVSGG GKLKANDISI PGDISSAAFF IVAATITPGS AIRLCRVGVN PTRLGVINLL KMMGADIEVT HYTEKNEEPT ADITVRHARL KGIDIPPDQV PLTIDEFPVL LIAAAVAQGK TVLRDAAELR VKETDRIAAM VDGLQKLGIA AESLPDGVII QGGTLEGGEV NSYDDHRIAM AFAVAGTLAK GPVRIRNCDN VKTSFPNFVE LANEVGMNVK GVRGRGGF  
PCR Forward Primer
 
PCR Backward Primer
 
Shipped To Lab
NU  
Shipped Date
Jul 20, 2010  
Inserted
May 05, 2009  

Related Expressions

       
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2010-05-24 2010-05-25 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2013-05-15 2013-05-16 Sam Light Northwestern University NU_default_cloning success E. coli TB IPTG production
2010-10-01 2010-10-02 Sam Light Northwestern University NU_native_expression success E. coli TB IPTG production
2009-04-29 2009-04-30 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay