Clone

Target

IDP02162  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Apr 27, 2009  

Experiment End Date

Apr 29, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

205  

Reference Sequence Used

MFYEVFKRLL DILLSFMGLL LLSPIFLIII FMIKKDSKGP IFFKQKRYGK DKQFFYIYKF RTMYVDTPKD MPTHMLQDPS KCITKVGGFL RKSSLDELPQ IINILKGEMS IVGPRPALWN QDDLIAQRDK YGANAVPVGL TGWAQINGRD ELPIPDKAKL DGDYVKNKST WFDLKCIFLT VFSVFAKKGV VEGGTGALGN KEDLK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTTTTATGAGGTTTTTAAAAGAT  

PCR Backward Primer

TTATCCACTTCCAATGCTACTTTAAATCCTCTTTGTTAC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 05, 2009