Clone

Target

IDP00164  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

256  

Reference Sequence Used

MYFMKSKNRF LLISLLSFST SVFADVNLYG PGGPHVPLIK VAESFEKSQS KRVNITFGPQ ATWNDKAKKN ADILFGASEH SALAIAEGHS ERFSKFNIHP VFMREAIILV KKGNPKNIKG MADLLKPGIG IVVNDGAGVS NTSGTAVWED SVGRMKNVEK LQAFRSNIHV FAPNSGSARK AFVDGEDIDA WITWVDWAIA NPTIGDMVRM EDEYRIYRDF NVVLAKNPSS EAIDFFDYLT KSKDAEAIFQ HYGWFK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTATTTCATGAAAAGTAAGAATC  

PCR Backward Primer

TTATCCACTTCCAATGTCACTTAAACCAGCCATAGTG  

Shipped To Lab

ANL  

Shipped Date

Jun 30, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008