Clone

Target

IDP00257  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2009  

Experiment End Date

Jul 24, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

394  

Reference Sequence Used

MTNCVTISQR EILKGVEGVT WFEVLLEKTS CITIAAQNRP SIDLWLDDVK QSSVMASKEF CMKVEVSETQ IAARCPTQGD ATLPMEGKDD YVCKKTFSDR GWGNGCALFG KGSIVGCAKV ACNTANVMKT HIYEQQAVQY VVGIEVHRGE VIKANVSDKV IKASFSAEAE KHTVEIPDYG SLDFTCRVVA SADLSNIRLL EVDGHYFNVH EDWLDDLPLP WRINAGPWRG MDKLVNFREP YAVKMVIMGY GDQRPAVLGA LDKAEEIKKV GDNYHLNGGH VSCKVSVAKL KLKGMTYVVC GGKFAWAKKP IATNHDTVAM EVTYTGNDTP CRVTVKNVKE NSDDQGTLIT TNPFVESNGA TIFLELEPVY GLSTIKVGDI TYQWNQQGSV IGKAV  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGACCAATTGTGTGACCATAT  

PCR Backward Primer

TTATCCACTTCCAATGCACAGCCTTCCCAATGACAC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 10, 2009