Clone

Target

IDP00258  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2009  

Experiment End Date

Jul 24, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

396  

Reference Sequence Used

MTNCVSIQKR DILRGSGSTT WFDVLLEKGS CVTIVADDRP TVDIWLDRIT HESPIAGREY CMRVDISGLK IATRCPTLGE AYLSEEHTED YVCKRGFSDR GWGNGCGLFG KGSIVGCVKT TCKSSGIAKS YSYDIPKVKY VISTEVHKGE LISGNVSASV VSATFSSEAE KHSMELEDYG RLEFTCRVVS GSNLGSVRIL EIDNHYFNVH EDWLLDLPLP WRIPDGHWHD LGKLIAFKEP HAVKMVVQAY GDQRASLLKS LVKAEEIAKS GNSYYLPGGH VDCRVSLVNL KLKGTTYPYC GDSFVWKRRP TATHHGTVAM EVTYQGTDVP CKVSVIVEKD GQNGGNAGSL ITSNPIITAQ GSSVFLELEV PLGFSTIKVG AAKQQWRQDG SSIGKAM  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGACCAATTGTGTTTCTATCC  

PCR Backward Primer

TTATCCACTTCCAATGCATAGCTTTTCCTATTGAACTG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 10, 2009