Clone

Target
IDP00259  
Clone Suffix
1
Experiment Start Date
Jul 22, 2009  
Experiment End Date
Jul 24, 2009  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
171  
Reference Sequence Used
MHLQYYAISL VSLVSLIDCH KLAFNFNLEI NGSDTHSTVD VYLDDSQIIT FDGKDIRPTI PFMIGDEIFL PFYKNVFSEF FSLFRRVPTS TPYEDLTYFY ECDYTDNKST FDQFYLYNGE EYTVKTQEAT NKNMWLTTSE FRLKKWFDGE DCIMHLRSLV RKMEDSKRNT G  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGCATCTACAATATTATGCGAT  
PCR Backward Primer
TTATCCACTTCCAATGCTAACCAGTGTTTCGTTTACTG