Clone

Target

IDP00279  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2009  

Experiment End Date

Jul 24, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

242  

Reference Sequence Used

MNEMGLLETT KRDLGMSKEP GVVSPTSYLD VDLHPASAWT LYAVATTVIT PMLRHTIENS TANVSLAAIA NQAVVLMGLD KGWPISKMDL GVPLLALGCY SQVNPLTLIA AVLLLVTHYA IIGPGLQAKA TREAQKRTAA GIMKNPTVDG IMTIDLDPVI YDSKFEKQLG QVMLLVLCAV QLLLMRTSWA LCEVLTLATG PITTLWEGSP GKFWNTTIAV SMANIFRGSY LAGAGLALSI MKS  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAATGAAATGGGACTGTTGG  

PCR Backward Primer

TTATCCACTTCCAATGTGATTTCATGATAGAAAGAGCAA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 10, 2009