Clone

Target

IDP90203  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2009  

Experiment End Date

Jul 24, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

579  

Reference Sequence Used

MLCDYDTKMF NDTILDFISS EMSSESFIIT MFNNNSSTNI DNMCHLYFKY IEVCPSSLLF RLFVECCDIN KLVEGTTPLH CYLMNERFES SVLKNLLKEY GMNTFNVRDS DGHIPLHKYL THDKVENDIF DMLSDSIDSF SEYKNILIHY IYSKLDSKPI TYYVLYKLLR KGADPNYADD EGNTFLHYFC IYMSAYEKMS LMHREKKFIK ELVKYGADIN KVNNIGNTPL HNYVSQYDHS PSIIFTLLSL GADLTIQNND RLTPIMEYIK CEDIEYNILL MLINWYESKY KKLEKEEGQH LLYLFIRHND SNDLNILSYL LKKFDMKNDE YYNDITPLHN ACIACNIDIM SYLVYIGCNI NLPTKDNKSI FDIISTQPNN IIYRNCIIYH IIRNGLDISL SVIKSLLSII PYLPTDHYVR YIITYCILMN NNFIKEYNNQ CLNHNYRELF VNFITFDYID NIVSDCVNDI NRLKQENYYN ILRYEDIKYY HLVNDIYVTN KTFPMYTDII ENCNLRMRRK YKLINTVIDK IYSISSVDNR ELSLLPPEII REIISKLSEY DLNTILYGRN HLKHYYKFN  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCTATGCGATTATGATACAAA  

PCR Backward Primer

TTATCCACTTCCAATGCTAGTTAAATTTATAATAATGTTTTAG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 10, 2009