Clone

Target

IDP90271  

Clone Suffix

301

Sequence Comments

Wildtype NS1 residues 1-208 (missing the last 11 residues of the full length NS1) was cloned as NdeI/BamHI cut PCR fragment into the NdeI/BamHI sites of pET28A(+).  

Experiment Start Date

Oct 02, 2009  

Experiment End Date

Oct 06, 2009  

Person

Chris Nelson  

Lab

Washington University  

Protocol ID

WU_basic_cloning_protocol_1  

Status

success  

Vector

pET28A(+)  

Sequence Start

1  

Sequence End

208  

Reference Sequence Used

MGSSHHHHHH SSGLVPRGSH MDSNTMSSFQ VDCFLWHIR K RFADNGLG DA PFLDRLR RDQ KSLKGR GNTL GLDIE TATLV GKQI VEWILK EES SETLRMT IA SVPTSRYL S DMTLEEMSR DWFMLMPRQK IIGPLCVRL D QAIMEKNI VL KANFSVI FNR LETLIL LRAF TEEGA IVGEI SPLP SLPGHT YED VKNAVGV LI GGLEWNGN T VRVSENIQ  

Reference Sequence Variance

 

Confirmed Protein Sequence

 

PCR Forward Primer

 

PCR Backward Primer

 

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 21, 2009