Clone

Target

IDP90271  

Clone Suffix

303

Sequence Comments

Wildtype NS1 residues 79-208 were cloned as a NdeI/BamHI cut PCR fragment into the NdeI/BamHI sites of pET28A(+). This region is expected to encode the effector domain of NS1.  

Experiment Start Date

Oct 02, 2009  

Experiment End Date

Oct 06, 2009  

Person

Chris Nelson  

Lab

Washington University  

Protocol ID

WU_basic_cloning_protocol_1  

Status

success  

Vector

pET28A(+)  

Sequence Start

79  

Sequence End

208  

Reference Sequence Used

MGSSHHHHHH SSGLVPRGSH MTIASVPTSR YLSDMTLEEM SR DWFMLMP RQKIIGPLCV RLD QAIMEK NIVLKANFSV IFNR LETLI LLRAFTEEGA IVGEI SPLP SLPGHTYEDV KNAVGV LIG GLEWNGNTVR VSENIQ  

Reference Sequence Variance

 

Confirmed Protein Sequence

 

PCR Forward Primer

 

PCR Backward Primer

 

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 21, 2009