Clone

Target

IDP00690  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

421  

Reference Sequence Used

MDKIVIKGGN KLTGEVKVEG AKNAVLPILT ASLLASDKPS KLVNVPALSD VETINNVLTT LNADVTYKKD ENAVVVDATK TLNEEAPYEY VSKMRASILV MGPLLARLGH AIVALPGGCA IGSRPIEQHI KGFEALGAEI HLENGNIYAN AKDGLKGTSI HLDFPSVGAT QNIIMAASLA KGKTLIENAA KEPEIVDLAN YINEMGGRIT GAGTDTITIN GVESLHGVEH AIIPDRIEAG TLLIAGAITR GDIFVRGAIK EHMASLVYKL EEMGVELDYQ EDGIRVRAEG ELQPVDIKTL PHPGFPTDMQ SQMMALLLTA NGHKVVTETV FENRFMHVAE FKRMNANINV EGRSAKLEGK SQLQGAQVKA TDLRAAAALI LAGLVADGKT SVTELTHLDR GYVDLHGKLK QLGADIERIN D  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGATAAAATAGTAATCAAAGGT  

PCR Backward Primer

TTATCCACTTCCAATGTTAATCGTTAATACGTTCAATGTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008