Clone

Target

IDP90822  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Oct 28, 2009  

Experiment End Date

Oct 28, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

321  

Reference Sequence Used

MHVLIVKTSS MGDVLHTLPA LTDAMNAIPG IRFDWVVEEG FSQIPSWHPA VDKVIPVAIR RWRKNWFGSD TRQERCDFKR VVQQRSYDRV IDAQGLIKSA ALITRIAKGT KHGPDCKSAR EPFASWFYNC RHEIDTKQHA VERIRQLFAK SLGYDKPESY GDYAIAQRFL NQLPADAGQY LVFLHATTRD NKHWPESHWV QLIELVQPTG LKIKLPWGAE HEHQRALRLA EHFPHVEVLP KLSLQQVAEV LAGAKAVVSV DTGLSHLTAA LDRPNITLFG PTDPGLIGGY GKNQIAVISE QKKMDSLSAP TIMARLETLI S  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MHVLIVKTSS MGDVLHTLPA LTDAMNAIPG IRFDWVVEEG FSQIPSWHPA VDKVIPVAIR RWRKNWFGSD TRQERCDFKR VVQQRSYDRV IDAQGLIKSA ALITRIAKGT KHGPDCKSAR EPFASWFYNC RHEIDTKQHA VERIRQLFAK SLGYDKPESY GDYAIAQRFL NQLPADAGQY LVFLHATTRD NKHWPESHWV QLIELVQPTG LKIKLPWGAE HEHQRALRLA EHFPHVEVLP KLSLQQVAEV LAGAKAVVSV DTGLSHLTAA LDRPNITLFG PTDPGLIGGY GKNQIAVISE QKKMDSLSAP TIMARLETLI S  

PCR Forward Primer

TACTTCCAATCCAATGCGATGCACGTATTAATCGTTAAAAC  

PCR Backward Primer

TTATCCACTTCCAATGCTATGAAATGAGTGTTTCTAATC  

Shipped To Lab

ANL  

Shipped Date

May 03, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 04, 2009