Clone

Target

IDP90831  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Oct 28, 2009  

Experiment End Date

Oct 28, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

360  

Reference Sequence Used

MLVWLAEYLV KFYSGFNVFS YLTFRAIVSL LTALFISLWM GPHLIAWLQK LQIGQVVRND GPESHFSKRG TPTMGGLMIL FSITISVLMW AYPSNPYVWC VLFILIGYGI VGFIDDYRKV VRKNTKGLIA RWKYFWQSII ALAAAFTMYS IGKDTSATEL VVPFFKDIMP QLGLLYVLLA YFVIVGTSNA VNLTDGLDGL AIMPTVFVAA GFALVAWATG NVNFAAYLHI PYLRHAGELV IVCTAIVGAG LGFLWFNTYP AQVFMGDVGS LALGGALGTI AVLLRQEFLL VIMGGVFVVE TLSVILQVGS FKLRGQRIFR MAPIHHHYEL KGWPEPRVIV RFWIISLMLV LIGLATLKVR  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTTAGTTTGGTTGGCTGAAT  

PCR Backward Primer

TTATCCACTTCCAATGTTACCGCACCTTCAGCGTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 04, 2009