Clone

Target

IDP90905  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Oct 28, 2009  

Experiment End Date

Oct 28, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

346  

Reference Sequence Used

MSGNNPSLSY KDAGVDIDAG NALVERIKGA VKRTRRPEVM GGLGGFGALC ELPTKYKHPV LVSGTDGVGT KLRLALDMKK HDTIGIDLVA MCVNDLIVQG AEPLFFLDYY ATGKLDVDTA AEVISGIADG CLQAGCALIG GETAEMPGMY EGEDYDVAGF CVGVVEKEEI IDGSKVQVGD ALIAVGSSGP HSNGYSLVRK ILEVSKADKN ERLAGKTIGE HLLAPTKIYI KSGLKLIAEH DIHAISHITG GGFWENIPRV LPEGTKAVID GKSWEWPVIF QWLQEKGNVT THEMYRTFNC GVGLIIALPK DQANAAVALL QAEGETAWVI GEIAAANSNE AQVEIN  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MSGNNPSLSY KDAGVDIDAG NALVERIKGA VKRTRRPEVM GGLGGFGALC ELPTKYKHPV LVSGTDGVGT KLRLALDMKK HDTIGIDLVA MCVNDLIVQG AEPLFFLDYY ATGKLDVDTA AEVISGIADG CLQAGCALIG GETAEMPGMY EGEDYDVAGF CVGVVEKEEI IDGSKVQVGD ALIAVGSSGP HSNGYSLVRK ILEVSKADKN ERLAGKTIGE HLLAPTKIYI KSGLKLIAEH DIHAISHITG GGFWENIPRV LPEGTKAVID GKSWEWPVIF QWLQEKGNVT THEMYRTFNC GVGLIIALPK DQANAAVALL QAEGETAWVI GEIAAANSNE AQVEIN  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGCGGTAATAATCCATCTC  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTTGATCTCTACCTGTGC  

Shipped To Lab

ANL  

Shipped Date

May 03, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 04, 2009