Clone

Target

IDP04054  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Nov 17, 2009  

Experiment End Date

Nov 30, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

190  

Reference Sequence Used

MRIIRPQQLV VLKSSYQIGH ESHMGISVVA GCYLSKPEHM VTESQIWQAW KAAPLSFRML DSAEPKPFAE FLLAGHAGIG EEVTSLSAEV SVGSLTRRWC IEGESNKTGL VIKPFLRMSM DHTQSWGGKG CKENPLGRGY NDERKPTIMS LGLDGSAIVR SPLASPSPVP HDFQLRKVHI NEVASTMTDP  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MRIIRPQQLV VLKSSYQIGH ESHMGISVVA GCYLSKPEHM VTESQIWQAW KAAPLSFRML DSAEPKPFAE FLLAGHAGIG EEVTSLSAEV SVGSLTRRWC IEGESNKTGL VIKPFLRMSM DHTQSWGGQR L*GESTGTWV *R*AQTDDYV FRP*RLCYRS LTAGVSVTCA S*LPTA*SAY Q*SRLDHDRS L  

PCR Forward Primer

TACTTCCAATCCAATGCGATGCGCATCATTCGCCCGC  

PCR Backward Primer

TTATCCACTTCCAATGTTAAGGATCGGTCATGGTCG  

Shipped To Lab

UT  

Shipped Date

Jun 19, 2012  

Secondary Shipped To Lab

UT  

Secondary Shipped Date

Jun 19, 2012  

Inserted

Dec 03, 2009