Clone

Target

IDP04097  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Nov 17, 2009  

Experiment End Date

Nov 30, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

229  

Reference Sequence Used

MYYVNKNKRK NIDFKKYLII TLLMIIACFL IFIYVFDKII APTVLLVSDS EMQAKTLDII NTNIAEVYTE KFNYNDVIKV QKDSSGAIKM INADTIKLNE LATEVAIKSQ RDIKEVGSIG VKMPIGYITK NNIMSYWGPS ITVKMEPIGR VVVTYESLFE SAGINQTRHK ILVNVKTNLK IILPLQSKEV EVVNQIPISD TVIVGEVPNS MWGGNMLQGI NEESREDTN  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTATTATGTAAATAAAAATAAACG  

PCR Backward Primer

TTATCCACTTCCAATGCTAATTTGTATCTTCTCTACTTTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Dec 03, 2009