Clone

Target

IDP04169  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Nov 17, 2009  

Experiment End Date

Nov 30, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

419  

Reference Sequence Used

MSLLAIGINH NTASVELREK VAFGPEKLSL ALNQLSTSSH VKGGVILSTC NRTEIYCDVR SASKNKVIEW LSQFHQVSLD ELKPSLYVHE EQAAIRHLMR VACGLDSLVL GEPQILGQVK QAYAEARENH AVNPATEKLF QKAFSVAKRV RTETEIGGSA VSVAYAACTL AKHIFESLAD ATVLLVGAGE TIELVAKHLA GHHCKRMIVA NRTRERALSL AQQFGADVIA LNEIPDYLAQ ADIVISSTAS PLPIIGKGMV ESALKARRHQ PMLLVDIAVP RDIEPQVGKL NDAYLYSVDD LQSIVDSNIE QRKVEAIQAE AIVSEESATF MSWMRSLQAV DSIRDYRKQA NEAREELLNK SLQALAAGGD PEKLLIELSN KLTNKLIHTP TRALQTAAEQ GEPAKLAVIR QSLGLDDLN  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTCTTTGCTTGCCATTGGA  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTTCAGATCGTCAAGACC  

Shipped To Lab

UT  

Shipped Date

Jan 20, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Dec 03, 2009