Clone

Target
IDP04311  
Clone Suffix
1 - The clone is available in BEIR but it is out of stock.
Experiment Start Date
Nov 17, 2009  
Experiment End Date
Nov 30, 2009  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
96  
Reference Sequence Used
MLRLDLQFFA SKKGVGSTKN GRDSQSKRLG AKRADGQTVS GGSILYRQRG TKIYPGVNVG RGGDDTLYAK VDGVVRFERL GRDRKQVSVY PVAQEA  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MLRLDLQFFA SKKGVGSTKN GRDSQSKRLG AKRADGQTVS GGSILYRQRG TKIYPGVNVG RGGDDTLYAK VDGVVRFERL GRDRKQVSVY PVAQEA  
PCR Forward Primer
TACTTCCAATCCAATGCGATGTTAAGATTAGATCTTCAGTTT  
PCR Backward Primer
TTATCCACTTCCAATGTTATGCTTCTTGAGCAACAGG  
Shipped To Lab
ANL  
Shipped Date
Dec 29, 2009  
Secondary Shipped To Lab
UT  
Secondary Shipped Date
Sep 19, 2011  
Inserted
Dec 03, 2009  

Related Expressions

       
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2009-11-30 2009-12-07 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2010-02-02 2010-02-04 Magdalena Makowska-Grzyska Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal + semet 0.5 mM IPTG production
2010-02-02 2010-02-04 Magdalena Makowska-Grzyska Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal + semet 0.5 mM IPTG production
2012-01-30 Maite Garcia University of Calgary Expression_protocol_Toronto success E. coli 15N 2X M9 none production