Clone

Target

IDP04313  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Nov 17, 2009  

Experiment End Date

Nov 30, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

258  

Reference Sequence Used

MNVGEFDGKT VLVTGAAQGI GSVVAKMFLE RGATVIAVDQ NGEGLNVLLN QNETRMKIFH LDVSDSNAVE DTVKRIENDI APIDILVNVA GVLRMGAIHS LSDEDWNKTF SVNSTGVFYM SRAVSKHMMQ RKSGAIVTVG SNAANTPRVE MAAYAASKAA TTMFMKCLGL ELAAYNIRCN LVSPGSTETE MQRLLWADEN GAKNIIAGSQ NTYRLGIPLQ KIAQPSEITE AVLFLASDKA SHITMHNLCV DGGATLGV  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MNVGEFDGKT VLVTGAAQGI GSVVAKMFLE RGATVIAVDQ NGEGLNVLLN QNETRMKIFH LDVSDSNAVE DTVKRIENDI APIDILVNVA GVLRMGAIHS LSDEDWNKTF SVNSTGVFYM SRAVSKHMMQ RKSGAIVTVG SNAANTPRVE MAAYAASKAA TTMFMKCLGL ELAAYNIRCN LVSPGSTETE MQRLLWADEN GAKNIIAGSQ NTYRLGIPLQ KIAQPSEITE AVLFLASDKA SHITMHNLCV DGGATLGV  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAACGTAGGGGAATTTGATG  

PCR Backward Primer

TTATCCACTTCCAATGTTAAACTCCTAATGTGGCTCC  

Shipped To Lab

UT  

Shipped Date

Jan 20, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Dec 03, 2009