Clone

Target

IDP00775  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

490  

Reference Sequence Used

MNYILGTILE SKITGVEKAQ INRLKLFKQH GISSKCVYVK WNPYSYTYAK QHQIENDVFT MYDYFQKAIN YKKTKQVNWI QYWEKSCRYT LKFVENSNDV RIYDEEQFVM YAHFLDKQYH QLNYVNYFDH KRRKVKRELY DGRGFLSCSR ILGEGQRIVL ENYYTPNGEI VIQKYFDDIK GKNTLTKVIL NEDQHQQFFD TEDELVQYFL HQLCKNNDQI ILDRPHELGN VIAGLNQSIP VIVVLHSTHL SGAGNGIKSF YKTVFNNLTR YKAIVVSTEK QCQDISQYIE NKIPVINIPV GYVANLKYQF DINQKEKNHI ISIARLVENK QIKHQIEVIK QLVTKHPNIQ LNIYGHGNGL SEYRQLVEDY HLSEHVKFHG FKTHINEEIA KAELMLSTSK MEGFGLAILE SLSVGTPVIS YDVDYGPSEL IQDGFNGYLV PQGDINQMVE KVDQLLNNTQ KLQQFSINSI ESAQQYNATT ISTKWQNILN  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAATTACATTTTAGGAACAATTT  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTTTAAAATATTTTGCCACTTT  

Shipped To Lab

UT  

Shipped Date

Jul 16, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008