Clone

Target

IDP04480  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Nov 17, 2009  

Experiment End Date

Nov 30, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

337  

Reference Sequence Used

MKIIYDGIIN SLQNMGGVTV YFSELVSRLP ATDFYWYSYA DEISGFGVGN VKIKSRLLER YRNFSLDTIN YNSLSVFHSS YYRLPDFDIP IVTTVHDFTY EKFVNGPAKW VHSWQKNRAV NNSDLIICVS ENTAKDLQMY CSVPSNKIRV IYNGVSDKYH YIKGVKITTN KVIFVGARGG YKNFDIAVKA ISKTPHLELS VVGGGAFTSK ELSLLNHYLP GRYHGLGRLS DEALNEAYNS AYALLYPSSY EGFGIPILEA MSAGCPVISV NVSSIPEVAG DAAILVQKPT IDELVDGLLA VESERSKLIG YGMKQAAKFS WDKCYQETLD VYKELNK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAATTATTTATGATGGAATAAT  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTGTTCAATTCTTTATAAACAT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Dec 03, 2009