Clone

Target

IDP04530  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Nov 17, 2009  

Experiment End Date

Nov 30, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

490  

Reference Sequence Used

MVPVVALVGR PNVGKSTLFN RLTRTRDALV ADFPGLTRDR KYGRAEVEGR EFICIDTGGI DGTEDGVETR MAEQSLLAIE EADVVLFMVD ARAGLMPADE AIAKHLRSRE KPTFLVANKT DGLDPDQAVV DFYSLGLGEI YPIAASHGRG VLSLLEHVLL PWMDDVAPQE EVDEDAEYWA QFEAEQNGEE APEDDFDPQS LPIKLAIVGR PNVGKSTLTN RILGEERVVV YDMPGTTRDS IYIPMERDER EYVLIDTAGV RKRGKITDAV EKFSVIKTLQ AIEDANVVLL VIDAREGISD QDLSLLGFIL NSGRSLVIVV NKWDGLSQEV KEQVKETLDF RLGFIDFARV HFISALHGSG VGNLFESVRE AYDSSTRRVS TAMLTRIMTM AVEDHQPPLV RGRRVKLKYA HAGGYNPPIV VIHGNQVKDL PDSYKRYLMN YFRKSLEVMG TPIRIQFKEG ENPYANKRNT LTPTQMRKRK RLMKHIKKSK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGTACCTGTGGTCGCGC  

PCR Backward Primer

TTATCCACTTCCAATGTTACTTGCTTTTCTTGATATGTTT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Dec 03, 2009