Clone

Target

IDP04545  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Nov 17, 2009  

Experiment End Date

Nov 30, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

178  

Reference Sequence Used

MLYDFKVKDI EGNEVSLGEY KGKVLLIVNT ATGCGFTPQY EGLEVLYKKY HDKGFEILDF PCNQFFEQAP GSNEEIVGFC KLNYGTTFKT FAKVEVNGEN ACELYKFLKK EAPMAKEDET SLGFYDKLKG LGFTTEGEEI KWNFTKFLID KNGEVVARFA PTFEPEKLDE LIEELLEE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MLYDFKVKDI EGNEVSLGEY KGKVLLIVNT ATGCGFTPQY EGLEVLYKKY HDKGFEILDF PCNQFFEQAP GSNEEIVGFC KLNYGTTFKT FAKVEVNGEN ACELYKFLKK EAPMAKEDET SLGFYDKLKG LGFTTEGEEI KWNFTKFLID KNGEVVARFA PTFEPEKLDE LIEELLEE  

PCR Forward Primer

TACTTCCAATCCAATGCGATGCTATATGATTTTAAAGTTAAGG  

PCR Backward Primer

TTATCCACTTCCAATGTTATTCTTCTAATAATTCTTCTATTA  

Shipped To Lab

ANL  

Shipped Date

Dec 29, 2009  

Secondary Shipped To Lab

UT  

Secondary Shipped Date

Sep 19, 2011  

Inserted

Dec 03, 2009