Clone

Target
IDP04666  
Clone Suffix
1 - The clone is available in BEIR but it is out of stock.
Experiment Start Date
Nov 17, 2009  
Experiment End Date
Nov 30, 2009  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
319  
Reference Sequence Used
MKKKKIGLLV MAYGTPDSLD EVEAYYTHIR HGRKPSEEAL QDLIGRYKAI GGISPLAKIT KEQAHKLTDS MNNMFTEYEF NCYLGLKHTA PFIEDAVEEM KRDGIEQAIS IVLAPHYSTF SIKAYNERAI RLSEEIGGPV IKPIDQWYDE PKFISYWADQ IKETFTKIED KEKAVVIFSA HSLPEKIIAA GDPYVEQLQH TADLIAAAAN IQNYTIGWQS AGNTPDPWIG PDVQDLTRDL FEEYRYESFI YCPVGFVAEH LEVLYDNDYE CKVVTDELNA AYFRPNMPNA QSTFIDCLAT IVSRKMKEIV DKELILNNN  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MKKKKIGLLV MAYGTPDSLD EVEAYYTHIR HGRKPSEEAL QDLIGRYKAI GGISPLAKIT KEQAHKLTDS MNNMFTEYEF NCYLGLKHTA PFIEDAVEEM KRDGIEQAIS IVLAPHYSTF SIKAYNERAI RLSEEIGGPV IKPIDQWYDE PKFISYWADQ IKETFTKIED KEKAVVIFSA HSLPEKIIAA GDPYVEQLQH TADLIAAAAN IQNYTIGWQS AGNTPDPWIG PDVQDLTRDL FEEYRYESFI YCPVGFVAEH LEVLYDNDYE CKVVTDELNA AYFRPNMPNA QSTFIDCLAT IVSRKMKEIV DKELILNNN  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAAAAAGAAAAAAATAGGTTTAC  
PCR Backward Primer
TTATCCACTTCCAATGTTAATTATTATTCAAAATTAGTTCTTT  
Shipped To Lab
ANL  
Shipped Date
Dec 29, 2009  
Inserted
Dec 03, 2009  

Related Expressions

                   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2009-11-30 2009-12-07 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2011-05-19 2011-05-20 Rob Solberg University of Virginia uva_expression_semet_1 success E. coli semet IPTG production
2012-01-04 2012-01-05 Rob Solberg University of Virginia uva_expression_semet_1 success E.coli LB IPTG production
2012-01-03 2012-01-05 Katarzyna Handing University of Virginia uva_expression_native_1 success E.coli LB 1M IPTG production
2012-01-30 2012-01-30 Katarzyna Handing University of Virginia uva_expression_native_1 success E.coli LB 0.1 mM IPTG production
2012-01-16 2012-01-18 Katarzyna Handing University of Virginia uva_expression_native_1 success E.coli LB IPTG production
2012-02-01 2012-02-03 Katarzyna Handing University of Virginia uva_expression_native_1 success E.coli LB 0.1 mM IPTG production
2011-10-29 2011-10-31 Katarzyna Handing University of Virginia uva_expression_semet_1 success E.coli SelMet IPTG production
2014-02-26 2014-02-27 Karol Szlachta University of Virginia uva_histag_purification_1.1 success Ecoli LB 0.5 mM IPTG production
2010-02-02 2010-02-04 Natalia Maltseva Argonne National Laboratory standard expression_ANL success E. coli M9 minimal + semet 0.5 mM IPTG production