Clone

Target

IDP90842  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Oct 28, 2009  

Experiment End Date

Dec 14, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

297  

Reference Sequence Used

MPPRRYNPDT RRDELLERIN LDIPGAVAQA LREDLGGTVD ANNDITAKLL PENSRSHATV ITRENGVFCG KRWVEEVFIQ LAGDDVTIIW HVDDGDVINA NQPLFELEGP SRVLLTGERT ALNFVQTLSG VASKVRHYVE LLEGTNTQLL DTRKTLPGLR SALKYAVLCG GGANHRLGLS DAFLIKENHI IASGSVRQAV EKASWLHPDA PVEVEVENLE ELDEALKAGA DIIMLDNFET EQMREAVKRT NGKALLEVSG NVTDKTLREF AETGVDFISV GALTKHVQAL DLSMRFR  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCCGCCTCGCCGCTAC  

PCR Backward Primer

TTATCCACTTCCAATGTTAGCGAAAACGCATTGAAAGG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jan 08, 2010