Clone

Target

IDP00141  

Clone Suffix

5

Sequence Comments

 

Experiment Start Date

Apr 15, 2010  

Experiment End Date

Apr 15, 2010  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

105  

Reference Sequence Used

MVRYGVLLAL IAVTYSYAGE DPTAPLGWVS PAKPELQKPS YPVPTLQSIV CAEGRSCYAI IEQRVVAQGD VVNGYRVATI HPDQVKLTRA GKEWRLALFT LDIKQ  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGTTAGATATGGTGTGCTC  

PCR Backward Primer

TTATCCACTTCCAATGTTATTGTTTTATATCGAGCGTAAA  

Shipped To Lab

ANL  

Shipped Date

Apr 29, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 03, 2010