Clone

Target

IDP00155  

Clone Suffix

5 - The clone is available in BEIR and it is in stock.

Sequence Comments

 

Experiment Start Date

Apr 15, 2010  

Experiment End Date

Apr 15, 2010  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

575  

Reference Sequence Used

MPINKLRKRL GDLLVEEGIV SEAQLEQALN AQKNTGRRLG DTLISLGFLS ETQLLNFLAQ QLSLPVIDLS RAHVDIDAVP LLPEVHARRL RALVIGRSGD TLRIAMSDPA DLFAQEALLN QLPDYGFEFV IAPEKQLVDG FDRYYRRTKE IVSFAEQLHA EHKTNDSFDF EITDSDSDEV TVVKLINSLF EDAIQVGASD IHIEPDANVL RLRQRIDGVL HETLLHEVNI ASALVLRLKL MANLDISEKR LPQDGRFNIR VKGQSVDIRM STMPIQHGES VVMRLLNQSA GVRKLEESGI PPHLLLRLRH QLKRPHGMIL VTGPTGSGKT TTLYGALNEL NTSGKKIITA EDPVEYRISR INQVQVNPKI NLDFSTLLRT FLRQDPDIIL VGEMRDQETV EIGLRAALTG HLVLSTLHTN DAVDSALRLI DMGAPGYLVA SAVRAVVAQR LVRKVCPDCS GHDEVDEARR QWLVTRFPNQ AAAKFTRGRG CQNCNLTGYR GRIGVFEMLE LDQPMMDCLR ANDAVAFSKA ARSNTDYKPL LASAMELALQ GIVSLDEVMS LGEGDSSGLV EPIYL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MPINKLRKRL GDLLVEEGIV SEAQLEQALN AQKNTGRRLG DTLISLGFLS ETQLLNFLAQ QLSLPVIDLS RAHVDIDAVP LLPEVHARRL RALVIGRSGD TLRIAMSDPA DLFAQEALLN QLPDYGFEFV IAPEKQLVDG FDRYYRRTKE IVSFAEQLHA EHKTNDSFDF EITDSDSDEV TVVKLINSLF EDAIQVGASD IHIEPDANVL RLRQRIDGVL HETLLHEVNI ASALVLRLKL MANLDISEKR LPQDGRFNIR VKGQSVDIRM STMPIQHGES VVMRLLNQSA GVRKLEESGI PPHLLLRLRH QLKRPHGMIL VTGPTGSGKT TTLYGALNEL NTSGKKIITA EDPVEYRISR INQVQVNPKI NLDFSTLLRT FLRQDPDIIL VGEMRDQETV EIGLRAALTG HLVLSTLHTN DAVDSALRLI DMGAPGYLVA SAVRAVVAQR LVRKVCPDCS GHDEVDEARR QWLVTRFPNQ AAAKFTRGRG CQNCNLTGYR GRIGVFEMLE LDQPMMDCLR ANDAVAFSKA ARSNTDYKPL LASAMELALQ GIVSLDEVMS LGEGDSSGLV EPIYL  

PCR Forward Primer

TACTTCCAATCCAATGCGATGCCAATTAATAAACTGCGTAA  

PCR Backward Primer

TTATCCACTTCCAATGCTACAGATAAATCGGTTCAACC  

Shipped To Lab

ANL  

Shipped Date

Apr 29, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 03, 2010