Clone

Target
IDP00163  
Clone Suffix
6
Experiment Start Date
Apr 15, 2010  
Experiment End Date
Apr 15, 2010  
Person
Jason Stam  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
276  
Reference Sequence Used
MIGKKSFQTN VYRMSKFDTY IFNNLYINDY KMFWIDSGIA KLIDKNCLVS YEINSSSIIL LKKNSIQRFS LTSLSDENIN VSVITISDSF IRSLKSYILG DLMIRNLYSE NKDLLLWNCE HNDIAVLSEV VNGFREINYS DEFLKVFFSG FFSKVEKKYN SIFITDDLDA MEKISCLVKS DITRNWRWAD ICGELRTNRM ILKKELESRG VKFRELINSI RISYSISLMK TGEFKIKQIA YQSGFASVSY FSTVFKSTMN VAPSEYLFML TGVAEK  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGATTGGGAAAAAATCTTTTCAA  
PCR Backward Primer
TTATCCACTTCCAATGTTATTTTTCTGCAACTCCTGTC  

Related Expressions

 
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2010-04-15 2010-04-19 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay