Clone

Target

IDP00223  

Clone Suffix

5 - The clone is available in BEIR and it is in stock.

Sequence Comments

 

Experiment Start Date

Apr 15, 2010  

Experiment End Date

Apr 15, 2010  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

225  

Reference Sequence Used

MTDNTLTPQM LTGLSTDHLV VLSGAHRLQP QAAEAFQAMQ QAAKVAGFDL QPASTFRDFG RQLAIWNGKF RGERPVLDQD SQPIDIFSLD TAARCEAILR WSALPGTSRH HWGSDLDIYD PSRLPTGEKL QLEPWEYDAG GYFYPLTQWL SAHMAEFGFY RPFATDNGGV AVEPWHLSYR PLAEQAEHLL TPKILLAAWQ SQDVAGSEWL ASHLPMIFSR FIYLK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MTDNTLTPQM LTGLSTDHLV VLSGAHRLQP QAAEAFQAMQ QAAKVAGFDL QPASTFRDFG RQLAIWNGKF RGERPVLDQD SQPIDIFSLD TAARCEAILR WSALPGTSRH HWGSDLDIYD PSRLPTGEKL QLEPWEYDAG GYFYPLTQWL SAHMAEFGFY RPFATDNGGV AVEPWHLSYR PLAEQAEHLL TPKILLAAWQ SQDVAGSEWL ASHLPMIFSR FIYLK  

PCR Forward Primer

TACTTCCAATCCAATGCGATGACCGATAATACATTAACGC  

PCR Backward Primer

TTATCCACTTCCAATGCTATTTAAGGTAGATAAATCGTG  

Shipped To Lab

ANL  

Shipped Date

Apr 29, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 03, 2010