Clone

Target

IDP00536  

Clone Suffix

3 - The clone is available in BEIR and it is in stock.

Sequence Comments

 

Experiment Start Date

Apr 15, 2010  

Experiment End Date

Apr 15, 2010  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

535  

Reference Sequence Used

MTISLRRTGI LKFGIGLVAL TIAASVQAKT LVYCSEGSPE GFNPQLFTSG TTYDASSVPI YNRLVEFKIG TTEIEPSLAE RWEVSEDGKT YTFYLRKGVK WQDNKDFKPT RDFNADDVIY SFMRQKDDKN PYHKVSGGSY EYFQGMGMGD LITNVVKVDD NTVRFELTRP ESPFLADLAM DFASILSAEY ADNMLKAGTP EKVDLNPIGT GPFQLQQYQK DSRILYKAFP GFWGTKPKID RLVFSITPDA SVRYAKLQKN ECQIMPYPNP ADIARMKEDK TINLMEQPGL NVGYLSFNIE KKPLDNLKVR QALTMAVNKD AIIDAVYQGA GQAAKNLIPP TMWGYNDDVK DYAYDPAKAK ELLKEAGLPD GFSIDLWAMP VQRPYNPNAR RMAEMIQSDW AKIGVKAKIV TYEWGEYLKR AKDGEHETVM MGWTGDNGDP DNFFATLFSC DAAKQGSNYS KWCYKPFEDL IQPARAEADH DKRVALYKQA QVVMNEQAPA LIIAHSTVYE PVRKEVKGYV VDPLGKHHFD NVSLD  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MTISLRRTGI LKFGIGLVAL TIAASVQAKT LVYCSEGSPE GFNPQLFTSG TTYDASSVPI YNRLVEFKIG TTEIEPSLAE RWEVSEDGKT YTFYLRKGVK WQDNKDFKPT RDFNADDVIY SFMRQKDDKN PYHKVSGGSY EYFQGMGMGD LITNVVKVDD NTVRFELTRP ESPFLADLAM DFASILSAEY ADNMLKAGTP EKVDLNPIGT GPFQLQQYQK DSRILYKAFP GFWGTKPKID RLVFSITPDA SVRYAKLQKN ECQIMPYPNP ADIARMKEDK TINLMEQPGL NVGYLSFNIE KKPLDNLKVR QALTMAVNKD AIIDAVYQGA GQAAKNLIPP TMWGYNDDVK DYAYDPAKAK ELLKEAGLPD GFSIDLWAMP VQRPYNPNAR RMAEMIQSDW AKIGVKAKIV TYEWGEYLKR AKDGEHETVM MGWTGDNGDP DNFFATLFSC DAAKQGSNYS KWCYKPFEDL IQPARAEADH DKRVALYKQA QVVMNEQAPA LIIAHSTVYE PVRKEVKGYV VDPLGKHHFD NVSLD  

PCR Forward Primer

TACTTCCAATCCAATGCGATGACGATTTCCTTGAGAAGAA  

PCR Backward Primer

TTATCCACTTCCAATGTTAATCCAGGGACACGTTATC  

Shipped To Lab

ANL  

Shipped Date

Apr 29, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 03, 2010