Clone

Target

IDP00563  

Clone Suffix

3 - The clone is available in BEIR and it is in stock.

Sequence Comments

414: G -> D  

Experiment Start Date

Apr 15, 2010  

Experiment End Date

Apr 15, 2010  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

676  

Reference Sequence Used

MLRSTSDRFR WSSLSLAIAC TLPLATQAAD TTTTQTSSKK HSTDTMVVTA TGNERSSFEA PMMVTVIEGN APTSQTAATA ADMLRQVPGL TVTGSGRTNG QDVVMRGYGK QGVLTLVDGV RQGTDTGHLN STFLDPALVK RIEIVRGPAA LLYGSGALGG VIAYETVDAA DMLQPGQNSG YRVYSSAATG DHSFGLGASA FGRTDDLDGI LSFGTRDIGN IRQSNGFNAP NDETISNVLA KGTWQIDSIQ SLSANLRYYN NSAIEPKNPQ TSAPSSTNVM TNRSTIQRDA QLRYNIKPLD QEWLNATAQV YYSEVEINAR PQGSAEEGRE QTTEGVKLEN RTRLFIESPA SHLLTYGTET YKQEQTPGGA TESFPQAKIR FSSGWLQDEI TLRDLPVSIL AGTRYDNYSG SSDGYADVDA DKWSSRGAIS ITPTDWLMLF GSYAQAFRAP TMGEMYNDSK HFAIPIRPGL TLTNYWVPNP NLKPETNETQ EYGFGLRFSD LLMAEDDLQF KVSYFDTKAK DYISTRVDMQ AMTTTSVNID QAKIWGWDAS MSYKTALFNW DLAYNRTRGK NQNTDEWLDT INPDTVTSIV DVPVANSGFS VGWIGTFANR SSRVSSSTPQ AGYGVNDFYV SYKGQEAFKG MTTTMLLGNV FEKEYYTPQG IPQDGRNVKF FVSYQW  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MLRSTSDRFR WSSLSLAIAC TLPLATQAAD TTTTQTSSKK HSTDTMVVTA TGNERSSFEA PMMVTVIEGN APTSQTAATA ADMLRQVPGL TVTGSGRTNG QDVVMRGYGK QGVLTLVDGV RQGTDTGHLN STFLDPALVK RIEIVRGPAA LLYGSGALGG VIAYETVDAA DMLQPGQNSG YRVYSSAATG DHSFGLGASA FGRTDDLDGI LSFGTRDIGN IRQSNGFNAP NDETISNVLA KGTWQIDSIQ SLSANLRYYN NSAIEPKNPQ TSAPSSTNVM TNRSTIQRDA QLRYNIKPLD QEWLNATAQV YYSEVEINAR PQGSAEEGRE QTTEGVKLEN RTRLFIESPA SHLLTYGTET YKQEQTPGGA TESFPQAKIR FSSGWLQDEI TLRDLPVSIL AGTRYDNYSG SSDDYADVDA DKWSSRGAIS ITPTDWLMLF GSYAQAFRAP TMGEMYNDSK HFAIPIRPGL TLTNYWVPNP NLKPETNETQ EYGFGLRFSD LLMAEDDLQF KVSYFDTKAK DYISTRVDMQ AMTTTSVNID QAKIWGWDAS MSYKTALFNW DLAYNRTRGK NQNTDEWLDT INPDTVTSIV DVPVANSGFS VGWIGTFANR SSRVSSSTPQ AGYGVNDFYV SYKGQEAFKG MTTTMLLGNV FEKEYYTPQG IPQDGRNVKF FVSYQW  

PCR Forward Primer

TACTTCCAATCCAATGCGATGCTTCGTTCCACTTCCGA  

PCR Backward Primer

TTATCCACTTCCAATGCTACCACTGATAACTCACGAA  

Shipped To Lab

ANL  

Shipped Date

Apr 29, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 03, 2010