Clone

Target
IDP00844  
Clone Suffix
3 - The clone is available in BEIR and it is in stock.
Experiment Start Date
Apr 15, 2010  
Experiment End Date
Apr 15, 2010  
Person
Jason Stam  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
174  
Reference Sequence Used
MQIYLSTLTE LDYDKSLNSI EESFDDNPET SWQARAKVKH LRKSPCYNFE LEVIAKNENN DVVGHVLLIE VEINSDDKTY YGLAIASLSV HPELRGQKLG RGLVQAVEER AKAQEYSTVV VDHCFDYFEK LGYQNAAEHD IKLEFGDAPL LVKYLWDNLT DAPHGIVKFP EHFY  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MQIYLSTLTE LDYDKSLNSI EESFDDNPET SWQARAKVKH LRKSPCYNFE LEVIAKNENN DVVGHVLLIE VEINSDDKTY YGLAIASLSV HPELRGQKLG RGLVQAVEER AKAQEYSTVV VDHCFDYFEK LGYQNAAEHD IKLEFGDAPL LVKYLWDNLT DAPHGIVKFP EHFY  
PCR Forward Primer
TACTTCCAATCCAATGCGATGCAAATATATTTAAGTACTTTAAC  
PCR Backward Primer
TTATCCACTTCCAATGTTAATAAAAATGTTCTGGAAATTTTA  
Shipped To Lab
UT  
Shipped Date
Apr 29, 2010  
Secondary Shipped To Lab
UT  
Secondary Shipped Date
Sep 19, 2011  
Inserted
May 03, 2010  

Related Expressions

         
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2010-04-15 2010-04-19 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2011-06-22 2011-06-23 Karolina Majorek University of Virginia uva_expression_semet_1 success E. coli SeMet IPTG production
2010-06-15 Olena Onopriyenko University of Calgary Expression_protocol_Toronto success E. coli Se-Met none production
2011-08-16 2011-08-17 Karolina Majorek University of Virginia uva_expression_semet_1 success E.coli SeMet IPTG production
2012-02-28 Maite Garcia University of Calgary Expression_protocol_Toronto success E. coli 15N 2X M9 none production