Clone

Target

IDP00834  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

502  

Reference Sequence Used

MTIYNINFGI GWASSGVEYA QAYRAKLLRR TNQDTKFVFL DFIQSENIQT LTQNIGFKDQ EVIWLYQYFS DISLAPTTYT LDDIERELGH DISFRERNGK IVRLYFNGKS SFVTCYLQNE QKDIVDRAEF VINSMLVRKD FYSYTRIFSE YYAPADNKAK LYMRQFYNQD GSIAYNEYID GDSSIYVFED AVLYNKTEFI AYFLQRLNLT RDDIVILDRA SDIGQAVLQH KGDSKVGVVI HADHYSNNMM SEQHILWNNY YEYQFSKAKY IDFFITATDI QNHMVCRQFE QYQGYRPRVY TIPVGSIDAL SYPTLSRKPY AMISASRLAN EKHIDWLVKA VIVAKRQVPE LTFDIYGEGS EKTRLRKIID THRAQDYIRL LGHVKLDEIY NDYELFLSAS TSEGFGLTLM EAVGSGLGMI GLNVNYGNPT FIRDGENGYL VPFDTDEDSV DDVIAKLAHA IVMYFNNGPQ TPHDISYEVA QQFMTQDIIL KWETLVQEVL HD  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGACAATATATAATATAAACTTTGG  

PCR Backward Primer

TTATCCACTTCCAATGTTAATCATGTAGCACCTCTTGA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008