Clone

Target

IDP01109  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Apr 15, 2010  

Experiment End Date

Apr 15, 2010  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

150  

Reference Sequence Used

MEATKRYLCL YLKESQEKFI SNWKKRILVH EHDPYKNEII KNGTHLLHVF TMYMREEINL QDIEDISKKI AQERMDAKVN IADFIYNTNE GKKEILNTLF LLNPTGQECK VVIEQINLFF DHLIYSTIYS YYKLKKEYIH SYYELKKKYN  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

EPVLPXQCDG SNKTILMFJP KRKPGEIYIK LEKTYFSSXX *SL*K*NN*K MERIYFMYSQ CICEKKLIYK TLKIFQKKLP KNVWMQK*IL QILYIIPMKE KKKY*IHYSF *IQLVKNAKW *LNR*ICSLI I*FIVRYIPI IN*KKNIFIH IMN*KRNITN IGSG*RIR  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGAAGCAACAAAACGATACT  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTTATATTTCTTTTTTAGTTCAT  

Shipped To Lab

UT  

Shipped Date

Apr 29, 2010  

Secondary Shipped To Lab

UT  

Secondary Shipped Date

Sep 19, 2011  

Inserted

May 03, 2010