Clone

Target

IDP01169  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Apr 02, 2010  

Experiment End Date

Apr 07, 2010  

Person

Hanna Tekleab  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

475  

Reference Sequence Used

MLTPISIEKE HIRLINLLHF INEQNRWFTI KELSDYLQVA DKTVRKYLKL LEDEIPPSWN LLVQKGKGIY LKKPLNESLS FVESKILRKS LNLQICEELV FKKNSMQSLA QKLHLQVGAL YPIINQINYD IQSSHLNIKK KPLEISGREQ DVRVFMLRLY CNIPNDYWPF PYINKQNITD LINKMEKILN VQMYTYSKHK LCVLFAITIS RLLSGNTIDN VSGLILVNKN DDHYKTVASI TSELQNSFGV TLHETEISFL ALALLLSLGN SITTDSNKTL TSYKKTIMPL AKEITKGIEH KLQLGINYDE SFLTYVVLII KKALDKNFIQ YYNYNIKFIR HIKQRHPNTF NTIQECISNL NYTVYSHFDC YEISLLTMHF ETQRMLFKNN PKKIYVYTSQ GCIHREYISA LLEKRYNGLI KIVRNTIINL TNESLQDMEI DIIISNVNLP IKNIPIVQIS EFPTERDFHE IKKII  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCTAACACCGATATCCATC  

PCR Backward Primer

TTATCCACTTCCAATGTTATATTATCTTTTTGATTTCATGAA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 03, 2010