Clone

Target
IDP01183  
Clone Suffix
3 - The clone is available in BEIR and it is in stock.
Experiment Start Date
Apr 15, 2010  
Experiment End Date
Apr 15, 2010  
Person
Jason Stam  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
94  
Reference Sequence Used
MLKPLGDRVV IELVQAEEKT ASGIVLPDTA KEKPQEGKVI AVGTGRVLEN GERVALEVAA GDLIIFSKYA GTEVKYEGTD YLILRESDIL AVIG  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MLKPLGDRVV IELVQAEEKT ASGIVLPDTA KEKPQEGKVI AVGTGRVLEN GERVALEVAA GDLIIFSKYA GTEVKYEGTD YLILRESDIL AVIG  
PCR Forward Primer
TACTTCCAATCCAATGCGATGCTAAAGCCATTAGGTGATC  
PCR Backward Primer
TTATCCACTTCCAATGTTAACCGATAACTGCTAAAATGT  
Shipped To Lab
ANL  
Shipped Date
Apr 29, 2010  
Secondary Shipped To Lab
UT  
Secondary Shipped Date
Sep 19, 2011  
Inserted
May 03, 2010  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2010-04-15 2010-04-19 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2012-03-06 Maite Garcia University of Calgary Expression_protocol_Toronto success E. coli 15N 2X M9 none production
2010-06-30 2010-07-02 Rory Mulligan Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 1 mM IPTG production