Clone

Target

IDP90953  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Apr 02, 2010  

Experiment End Date

Apr 07, 2010  

Person

Hanna Tekleab  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

827  

Reference Sequence Used

MLHKSDKRLL TFISLMPIAL IGIFTLVLNA IVIHENQTKV GSLIQSLYSD ALTQEKGRVK QQVDSIYEQI IYEDNLAEQT LKKSIQYRIE DAYSIIHFIY QQNEEKTKPE IIKLIADALR QTRFNDGRGY FFIYDMDGTN LMHPIFPEYE GKNLINFTDI HGKRVVEDHV KMMREKGEGF SQWWYPKPDN KDEAFEKIGY AKYFEPLDIY IGTGEYVIDV EEDIQQILLA WISEVRYGDD GYIFVFDHSG NILSHQDKSL INTNIYNKTD INPYEFMNTL NSKFDDNNSR FSKYLNRLDA FNNGSDSQLS YIKKYDKWDW VISGGVYTDT NKRLLEKKEK ILIQQNQEEL KKILLLTLTL ALILTLLSLS VSKYVAMRFN KFQDRICNDF KVLEKTKNKM QYMALHDGLT QLPNRVMLES RIEEEIVKAQ QSGKMIALMF VDLDDFKKVN DHYGHDVGDK LLTLISSRFS HFIEKDEIVA RFGGDEFVFC FSNLTSQQDA ELWVEKIQSV FENVFSINGK EITTGCSIGV AMYPKDATCS SELISKADIV LYKSKDVHKG ASLFFDKSID EQVSYEFSLE EELKNALDKR EVTLVYQPQI DTKTGEIYGV EVLSRWCNNK LGSVSPVKFI AVAEEIGMID QLGDFVLQQA CEDIQEVMPN GTNAIMLSVN ISPKQLIKKG FLSRIMKIVS RTGIDVSRIT FEITENILIS DLKSVSPILL ELQKLGFGIS LDDFGTGYSS LSYLNILPIT EIKIDRSFVS SLFTNDQSES LIKAIIAIGA SCQMTVVAEG VETKEQLEKL KEYQCDLVQG YYLDKPLSIS ELKAKYA  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCTGCATAAGTCTGATAAAAG  

PCR Backward Primer

TTATCCACTTCCAATGTTATGCATATTTTGCTTTTAATTCA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 03, 2010