Clone

Target

IDP00827  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

247  

Reference Sequence Used

MDYQTFEKVN KFINVKAYIF FLTQELKQQY KLSLKELLIL AYFYYKNEHS ISLKEIIGDI LYKQSDVVKN IKSLSKKGFI NKSRNEADER RIFVSVTPIQ RKKIACVINE LDKIIKGFNK ERDYIKYQWA PKYSKEFFIL FMNIMYSKDF LKYRFNLTFL DLSILYVISS RKNEILNLKD LFESIRFMYP QIVRSVNRLN NKGMLIKERS LADERIVLIK INKIQYNTIK SIFTDTSKIL KPRKFFF  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MDYQTFEKVN KFINVKAYIF FLTQELKQQY KLSLKELLIL AYFYYKNEHS ISLKEIIGDI LYKQSDVVKN IKSLSKKGFI NKSRNEADER RIFVSVTPIQ RKKIACVINE LDKIIKGFNK ERDYIKYQWA PKYSKEFFIL FMNIMYSKDF LKYRFNLTFL DLSILYVISS RKNEILNLKD LFESIRFMYP QIVRSVNRLN NKGMLIKERS LADERIVLIK INKIQYNTIK SIFTDTSKIL KPRKFFF  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGACTATCAAACTTTCGAAAA  

PCR Backward Primer

TTATCCACTTCCAATGTTAAAAGAAAAATTTTCTTGGTTTG  

Shipped To Lab

ANL  

Shipped Date

Jul 20, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008