Clone

Target
IDP05281  
Clone Suffix
1 The clone is not available in BEIR.
Experiment Start Date
Nov 06, 2010  
Experiment End Date
Nov 08, 2010  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
108  
Reference Sequence Used
MKRKVYLFCS FGMSTSLLAD KMQKVADEHN LPIEVEAFPI AEIDKIVEEK NPDCILLGPQ VKYMLKELKP KFEAQGKLID IINEVDYGTM NGEKVLKLAI KLIKSKQA  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MKRKVYLFCS FGMSTSLLAD KMQKVADEHN LPIEVEAFPI AEIDKIVEEK NPDCILLGPQ VKYMLKELKP KFEAQGKLID IINEVDYGTM NGEKVLKLAI KLIKSKQA  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAAAAGAAAAGTATATTTATTTTG  
PCR Backward Primer
TTATCCACTTCCAATGCTACGCTTGCTTAGATTTTATTA  
Shipped To Lab
UT  
Shipped Date
Mar 03, 2011  
Secondary Shipped To Lab
UT  
Secondary Shipped Date
Sep 19, 2011  
Inserted
Nov 24, 2010  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2010-11-17 2010-11-17 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2011-04-29 Marina Kudritska University of Calgary Expression_protocol_Toronto success E. coli LB none production
2012-02-27 Maite Garcia University of Calgary Expression_protocol_Toronto success E. coli 15N 2X M9 none production