Clone

Target

IDP05291  

Clone Suffix

1 The clone is not available in BEIR.

Sequence Comments

 

Experiment Start Date

Nov 06, 2010  

Experiment End Date

Nov 08, 2010  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

163  

Reference Sequence Used

MADKMAQNIT LQFVEEKDLE NLENVKLLFT EYSNSLNIDL CFQDFNNELK TLPGKYKKPS GSLILAFVDE NLAGCVALKK LEGKICELKR LYVRNQFRGL KIGKILLEEI IEEAKKFGYT HMRLDTLPSM KSAQGLYEKF GFYDIEPYTY NPIEGARYME LKL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MADKMAQNIT LQFVEEKDLE NLENVKLLFT EYSNSLNIDL CFQDFNNELK TLPGKYKKPS GSLILAFVDE NLAGCVALKK LEGKICELKR LYVRNQFRGL KIGKILLEEI IEEAKKFGYT HMRLDTLPSM KSAQGLYEKF GFYDIEPYTY NPIEGARYME LKL  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGCTGATAAAATGGCTCAAA  

PCR Backward Primer

TTATCCACTTCCAATGTTATAATTTTAATTCCATATATCTAG  

Shipped To Lab

UT  

Shipped Date

Mar 03, 2011  

Secondary Shipped To Lab

UT  

Secondary Shipped Date

Sep 19, 2011  

Inserted

Nov 24, 2010