Clone

Target

IDP00573  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

499  

Reference Sequence Used

MQGTKIRLMV GGLLLAAASN NVQAEALQPD PAWQQGKLDN GFSWQLLATP QRPSDRIELR LIVNTGSLSE NTQEVGFAHL LPRLALMSSA SFTPAQLQSL WQQGIDNERP LPPAITSYDF TLYSLSLPNN RPDLLKDALA WLSDTAGNLA VSEQTVNAAL NTATDPIATF PQNIQEPWWR YRLKGSSLIG HDPGQPVTQP VDVEKLKQFY QQWYTPDAMT LYVVGNVDSR SIAAQISKAF SELKGKRTAP AAVATLAPLP PEPVSLMNEQ AAQDTLSLMW DTPWHPIQDS MALSRYWRSD LAREALFWHI KQVLEKNNQK NLKLGFDCRV QYQRAQCAIH LNTPVENLTA NMTFVARELA ALRANGLSQA EFDALMTQKN DQLSKLFATY ARTDTDILMS QRLRSQQSGV VDIAPEQYQK LRQAFLSGLT LAELNRELKQ QLSQDTTLVL MQPKGEPEVN VKALQEIYNG IMAPQTVAEE EVAPAEAVET APVMPTTAQ  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MQGTKIRLMV GGLLLAAASN NVQAEALQPD PAWQQGKLDN GFSWQLLATP QRPSDRIELR LIVNTGSLSE NTQEVGFAHL LPRLALMSSA SFTPAQLQSL WQQGIDNERP LPPAITSYDF TLYSLSLPNN RPDLLKDALA WLSDTAGNLA VSEQTVNAAL NTATDPIATF PQNIQEPWWR YRLKGSSLIG HDPGQPVTQP VDVEKLKQFY QQWYTPDAMT LYVVGNVDSR SIAAQISKAF SELKGKRTAP AAVATLAPLP PEPVSLMNEQ AAQDTLSLMW DTPWHPIQDS MALSRYWRSD LAREALFWHI KQVLEKNNQK NLKLGFDCRV QYQRAQCAIH LNTPVENLTA NMTFVARELA ALRANGLSQA EFDALMTQKN DQLSKLFATY ARTDTDILMS QRLRSQQSGV VDIAPEQYQK LRQAFLSGLT LAELNRELKQ QLSQDTTLVL MQPKGEPEVN VKALQEIYNG IMAPQTVAEE EVAPAEAVET APVMPTTAQ  

PCR Forward Primer

TACTTCCAATCCAATGCGATGCAGGGCACCAAAATTCG  

PCR Backward Primer

TTATCCACTTCCAATGTTATTGCGCGGTTGTCGGC  

Shipped To Lab

UT  

Shipped Date

Jun 30, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008